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fix perm working solution  (Cytek Biosciences)


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    Cytek Biosciences fix perm working solution
    Fix Perm Working Solution, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fix+perm+working+solution/Flow+Cytometry+Perm+Buffer/pm38928413-260-23-26
    Average 95 stars, based on 1 article reviews
    fix perm working solution - by Bioz Stars, 2026-10
    95/100 stars

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    Incubation:

    Article Title: Trichinella spiralis Paramyosin Alleviates Collagen-Induced Arthritis in Mice by Modulating CD4 + T Cell Differentiation
    Article Snippet: Surface staining was then performed by adding anti-CD3e-PerCP5.5 (Clone: 145-2C11, TONBO, USA), anti-CD4-APC-Cy7 (Clone: GK1.5, TONBO, USA), and anti-CD25-PE-CF594 (Clone: PC61, BioLegend, San Diego, CA, USA). .. After 30 min of incubation at 4 °C in the dark, cells were washed with PBS, followed by the addition of Transcription Factor Fix/Perm working solution (TONBO, USA) and incubation at 4 °C for 1 h. After the Transcription Factor Perm working solution (TONBO, USA) was washed off, the cells were stained with intracellular/nuclear antibodies, namely anti-IFN-γ-violetFluor450 (Clone: XMG1.2, TONBO, USA), anti-IL-4-PE (Clone: 11B11, TONBO, USA), anti-IL-17A-PE-Cy7 (eBio17B7, eBioscience, USA), and anti-Foxp3-APC (Clone: 3G3, TONBO, USA), and incubated at 4 °C in the dark for 30 min. .. Following staining, cells were washed once with Transcription Factor Perm working solution and once with PBS before analysis using a BD FACS Symphony flow cytometer.

    Article Title: Trichinella spiralis Paramyosin Alleviates Collagen-Induced Arthritis in Mice by Modulating CD4 + T Cell Differentiation.
    Article Snippet: Surface staining was then performed by adding anti-CD3e-PerCP5.5 (Clone: 145-2C11, TONBO, USA), anti-CD4-APC-Cy7 (Clone: GK1.5, TONBO, USA), and anti-CD25-PECF594 (Clone: PC61, BioLegend, San Diego, CA, USA). .. After 30 min of incubation at 4 ◦C in the dark, cells were washed with PBS, followed by the addition of Transcription Factor Fix/Perm working solution (TONBO, USA) and incubation at 4 ◦C for 1 h. After the Transcription Factor Perm working solution (TONBO, USA) was washed off, the cells were stained with intracellular/nuclear antibodies, namely anti-IFN-γ-violetFluor450 (Clone: XMG1.2, TONBO, USA), anti-IL-4-PE (Clone: 11B11, TONBO, USA), anti-IL-17A-PE-Cy7 (eBio17B7, eBioscience, USA), and anti-Foxp3-APC (Clone: 3G3, TONBO, USA), and incubated at 4 ◦C in the dark for 30 min. ..

    Staining:

    Article Title: Trichinella spiralis Paramyosin Alleviates Collagen-Induced Arthritis in Mice by Modulating CD4 + T Cell Differentiation
    Article Snippet: Surface staining was then performed by adding anti-CD3e-PerCP5.5 (Clone: 145-2C11, TONBO, USA), anti-CD4-APC-Cy7 (Clone: GK1.5, TONBO, USA), and anti-CD25-PE-CF594 (Clone: PC61, BioLegend, San Diego, CA, USA). .. After 30 min of incubation at 4 °C in the dark, cells were washed with PBS, followed by the addition of Transcription Factor Fix/Perm working solution (TONBO, USA) and incubation at 4 °C for 1 h. After the Transcription Factor Perm working solution (TONBO, USA) was washed off, the cells were stained with intracellular/nuclear antibodies, namely anti-IFN-γ-violetFluor450 (Clone: XMG1.2, TONBO, USA), anti-IL-4-PE (Clone: 11B11, TONBO, USA), anti-IL-17A-PE-Cy7 (eBio17B7, eBioscience, USA), and anti-Foxp3-APC (Clone: 3G3, TONBO, USA), and incubated at 4 °C in the dark for 30 min. .. Following staining, cells were washed once with Transcription Factor Perm working solution and once with PBS before analysis using a BD FACS Symphony flow cytometer.

    Article Title: Trichinella spiralis Paramyosin Alleviates Collagen-Induced Arthritis in Mice by Modulating CD4 + T Cell Differentiation.
    Article Snippet: Surface staining was then performed by adding anti-CD3e-PerCP5.5 (Clone: 145-2C11, TONBO, USA), anti-CD4-APC-Cy7 (Clone: GK1.5, TONBO, USA), and anti-CD25-PECF594 (Clone: PC61, BioLegend, San Diego, CA, USA). .. After 30 min of incubation at 4 ◦C in the dark, cells were washed with PBS, followed by the addition of Transcription Factor Fix/Perm working solution (TONBO, USA) and incubation at 4 ◦C for 1 h. After the Transcription Factor Perm working solution (TONBO, USA) was washed off, the cells were stained with intracellular/nuclear antibodies, namely anti-IFN-γ-violetFluor450 (Clone: XMG1.2, TONBO, USA), anti-IL-4-PE (Clone: 11B11, TONBO, USA), anti-IL-17A-PE-Cy7 (eBio17B7, eBioscience, USA), and anti-Foxp3-APC (Clone: 3G3, TONBO, USA), and incubated at 4 ◦C in the dark for 30 min. ..



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    Anti-CD20 was administered to deplete B cells on days –7, 3, 18, 33, 48, and 63, and mice infected on day 0 and infected for the 2nd time on day 60 with C . difficile strain R20291. ( A ) Experimental design; ( B ) survival curve; ( C ) first infection weight loss; ( D ) first infection clinical scores; ( E ) reinfection weight loss; ( F ) reinfection clinical scores ( G ) epithelial damage scoring; ( H ) H&E stain. Scale bars: 118.7 μm (left) and 191.4 μm (right). ( I ) Toxin B–specific plasma IgG; ( J ) IgA; ( K ) cecal IgG; and ( L ) cecal IgA measured on day 10 after second infection. MLN and colon were harvested on day 10 after reinfection. Colonic ( M and N ) B cells (CD45 + CD3 – CD19 + ); ( O and P ) TH2 cells (CD45 + CD3 + CD4 + GATA3 + ); ( Q and R ) neutrophils; ( S – U ) Treg (CD45 + CD3 + CD4 + <t>FOXP3</t> + ), and TH17 (CD45 + CD3 + CD4 + RORγt + ) cells; and ( V and W ) MLN B cells. ( B ) Comparison made by log-rank test ( n = 26 in both groups). ( C – F ) Comparison made by 2-tailed Student’s t-test ( C and D n = 26, E and F n = 15). ( G ) Šídák’s multiple comparisons test was used. ( I – M , P , Q , T , U , and V ) A 2-tailed t test for normally distributed data and a Mann-Whitney test for nonnormally distributed data were used. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. The error bar indicates SEM.
    Foxp3 Fix/Perm Working Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fix+perm+working+solution/foxp3+transcription+factor+staining+buffer+set/pmc12043089-218-6-10
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    fix perm working solution  (Cytek Biosciences)
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    Anti-CD20 was administered to deplete B cells on days –7, 3, 18, 33, 48, and 63, and mice infected on day 0 and infected for the 2nd time on day 60 with C . difficile strain R20291. ( A ) Experimental design; ( B ) survival curve; ( C ) first infection weight loss; ( D ) first infection clinical scores; ( E ) reinfection weight loss; ( F ) reinfection clinical scores ( G ) epithelial damage scoring; ( H ) H&E stain. Scale bars: 118.7 μm (left) and 191.4 μm (right). ( I ) Toxin B–specific plasma IgG; ( J ) IgA; ( K ) cecal IgG; and ( L ) cecal IgA measured on day 10 after second infection. MLN and colon were harvested on day 10 after reinfection. Colonic ( M and N ) B cells (CD45 + CD3 – CD19 + ); ( O and P ) TH2 cells (CD45 + CD3 + CD4 + GATA3 + ); ( Q and R ) neutrophils; ( S – U ) Treg (CD45 + CD3 + CD4 + <t>FOXP3</t> + ), and TH17 (CD45 + CD3 + CD4 + RORγt + ) cells; and ( V and W ) MLN B cells. ( B ) Comparison made by log-rank test ( n = 26 in both groups). ( C – F ) Comparison made by 2-tailed Student’s t-test ( C and D n = 26, E and F n = 15). ( G ) Šídák’s multiple comparisons test was used. ( I – M , P , Q , T , U , and V ) A 2-tailed t test for normally distributed data and a Mann-Whitney test for nonnormally distributed data were used. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. The error bar indicates SEM.
    Fix Perm Working Solution, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    c tonbo fixation permeabilization working solution fix perm  (Cytek Biosciences)
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    Cytek Biosciences c tonbo fixation permeabilization working solution fix perm
    Anti-CD20 was administered to deplete B cells on days –7, 3, 18, 33, 48, and 63, and mice infected on day 0 and infected for the 2nd time on day 60 with C . difficile strain R20291. ( A ) Experimental design; ( B ) survival curve; ( C ) first infection weight loss; ( D ) first infection clinical scores; ( E ) reinfection weight loss; ( F ) reinfection clinical scores ( G ) epithelial damage scoring; ( H ) H&E stain. Scale bars: 118.7 μm (left) and 191.4 μm (right). ( I ) Toxin B–specific plasma IgG; ( J ) IgA; ( K ) cecal IgG; and ( L ) cecal IgA measured on day 10 after second infection. MLN and colon were harvested on day 10 after reinfection. Colonic ( M and N ) B cells (CD45 + CD3 – CD19 + ); ( O and P ) TH2 cells (CD45 + CD3 + CD4 + GATA3 + ); ( Q and R ) neutrophils; ( S – U ) Treg (CD45 + CD3 + CD4 + <t>FOXP3</t> + ), and TH17 (CD45 + CD3 + CD4 + RORγt + ) cells; and ( V and W ) MLN B cells. ( B ) Comparison made by log-rank test ( n = 26 in both groups). ( C – F ) Comparison made by 2-tailed Student’s t-test ( C and D n = 26, E and F n = 15). ( G ) Šídák’s multiple comparisons test was used. ( I – M , P , Q , T , U , and V ) A 2-tailed t test for normally distributed data and a Mann-Whitney test for nonnormally distributed data were used. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. The error bar indicates SEM.
    C Tonbo Fixation Permeabilization Working Solution Fix Perm, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fix/perm buffer working solution  (Becton Dickinson)
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    Anti-CD20 was administered to deplete B cells on days –7, 3, 18, 33, 48, and 63, and mice infected on day 0 and infected for the 2nd time on day 60 with C . difficile strain R20291. ( A ) Experimental design; ( B ) survival curve; ( C ) first infection weight loss; ( D ) first infection clinical scores; ( E ) reinfection weight loss; ( F ) reinfection clinical scores ( G ) epithelial damage scoring; ( H ) H&E stain. Scale bars: 118.7 μm (left) and 191.4 μm (right). ( I ) Toxin B–specific plasma IgG; ( J ) IgA; ( K ) cecal IgG; and ( L ) cecal IgA measured on day 10 after second infection. MLN and colon were harvested on day 10 after reinfection. Colonic ( M and N ) B cells (CD45 + CD3 – CD19 + ); ( O and P ) TH2 cells (CD45 + CD3 + CD4 + GATA3 + ); ( Q and R ) neutrophils; ( S – U ) Treg (CD45 + CD3 + CD4 + <t>FOXP3</t> + ), and TH17 (CD45 + CD3 + CD4 + RORγt + ) cells; and ( V and W ) MLN B cells. ( B ) Comparison made by log-rank test ( n = 26 in both groups). ( C – F ) Comparison made by 2-tailed Student’s t-test ( C and D n = 26, E and F n = 15). ( G ) Šídák’s multiple comparisons test was used. ( I – M , P , Q , T , U , and V ) A 2-tailed t test for normally distributed data and a Mann-Whitney test for nonnormally distributed data were used. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. The error bar indicates SEM.
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    Image Search Results


    Anti-CD20 was administered to deplete B cells on days –7, 3, 18, 33, 48, and 63, and mice infected on day 0 and infected for the 2nd time on day 60 with C . difficile strain R20291. ( A ) Experimental design; ( B ) survival curve; ( C ) first infection weight loss; ( D ) first infection clinical scores; ( E ) reinfection weight loss; ( F ) reinfection clinical scores ( G ) epithelial damage scoring; ( H ) H&E stain. Scale bars: 118.7 μm (left) and 191.4 μm (right). ( I ) Toxin B–specific plasma IgG; ( J ) IgA; ( K ) cecal IgG; and ( L ) cecal IgA measured on day 10 after second infection. MLN and colon were harvested on day 10 after reinfection. Colonic ( M and N ) B cells (CD45 + CD3 – CD19 + ); ( O and P ) TH2 cells (CD45 + CD3 + CD4 + GATA3 + ); ( Q and R ) neutrophils; ( S – U ) Treg (CD45 + CD3 + CD4 + FOXP3 + ), and TH17 (CD45 + CD3 + CD4 + RORγt + ) cells; and ( V and W ) MLN B cells. ( B ) Comparison made by log-rank test ( n = 26 in both groups). ( C – F ) Comparison made by 2-tailed Student’s t-test ( C and D n = 26, E and F n = 15). ( G ) Šídák’s multiple comparisons test was used. ( I – M , P , Q , T , U , and V ) A 2-tailed t test for normally distributed data and a Mann-Whitney test for nonnormally distributed data were used. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. The error bar indicates SEM.

    Journal: The Journal of Clinical Investigation

    Article Title: IL-33 protects from recurrent C . difficile infection by restoration of humoral immunity

    doi: 10.1172/JCI184659

    Figure Lengend Snippet: Anti-CD20 was administered to deplete B cells on days –7, 3, 18, 33, 48, and 63, and mice infected on day 0 and infected for the 2nd time on day 60 with C . difficile strain R20291. ( A ) Experimental design; ( B ) survival curve; ( C ) first infection weight loss; ( D ) first infection clinical scores; ( E ) reinfection weight loss; ( F ) reinfection clinical scores ( G ) epithelial damage scoring; ( H ) H&E stain. Scale bars: 118.7 μm (left) and 191.4 μm (right). ( I ) Toxin B–specific plasma IgG; ( J ) IgA; ( K ) cecal IgG; and ( L ) cecal IgA measured on day 10 after second infection. MLN and colon were harvested on day 10 after reinfection. Colonic ( M and N ) B cells (CD45 + CD3 – CD19 + ); ( O and P ) TH2 cells (CD45 + CD3 + CD4 + GATA3 + ); ( Q and R ) neutrophils; ( S – U ) Treg (CD45 + CD3 + CD4 + FOXP3 + ), and TH17 (CD45 + CD3 + CD4 + RORγt + ) cells; and ( V and W ) MLN B cells. ( B ) Comparison made by log-rank test ( n = 26 in both groups). ( C – F ) Comparison made by 2-tailed Student’s t-test ( C and D n = 26, E and F n = 15). ( G ) Šídák’s multiple comparisons test was used. ( I – M , P , Q , T , U , and V ) A 2-tailed t test for normally distributed data and a Mann-Whitney test for nonnormally distributed data were used. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. The error bar indicates SEM.

    Article Snippet: Cells were washed and resuspended in Foxp3 Fix/Perm WorkinFg Solution (ebiosciences, 00-5523-00) and incubated overnight at 4°C.

    Techniques: Infection, Staining, Clinical Proteomics, Comparison, MANN-WHITNEY

    IL-33 (0.75 μg) was administered i.p. on days –4 to 0 and mice infected on day 0. Mesenteric lymph nodes were harvested to analyze T helper cells before infection, and again on day 2 and day 6 after first infection. ( A – C ) TH17 cells (CD45 + CD3 + CD4 + RORgt + ) and Treg cells (CD45 + CD3 + CD4 + FOXP3 + ) on day 0 prior before infection ( n = 10); ( D – F ) on day 2 ( n = 20); ( G – I ) on day 6 after infection ( n = 24). ( J – M ) TFH cells were defined as germinal center (GC) TFH (CD45 + CD3 + CD4 + CD44 + PD1 high CXCR5 + ) and non-GC TFH cells (CD45 + CD3 + CD4 + CD44 + PD1-low CXCR5 + ) by flow cytometry. ( J and K ) TFH subsets on day 0 prior to infection ( n = 10); ( L and M ) TFH subsets on day 6 after infection( n = 28). A 2-tailed t test for normally distributed data and a Mann-Whitney test for nonnormally distributed data were used. * P < 0.05, ** P < 0.01, and *** P < 0.001. The error bar indicates SEM.

    Journal: The Journal of Clinical Investigation

    Article Title: IL-33 protects from recurrent C . difficile infection by restoration of humoral immunity

    doi: 10.1172/JCI184659

    Figure Lengend Snippet: IL-33 (0.75 μg) was administered i.p. on days –4 to 0 and mice infected on day 0. Mesenteric lymph nodes were harvested to analyze T helper cells before infection, and again on day 2 and day 6 after first infection. ( A – C ) TH17 cells (CD45 + CD3 + CD4 + RORgt + ) and Treg cells (CD45 + CD3 + CD4 + FOXP3 + ) on day 0 prior before infection ( n = 10); ( D – F ) on day 2 ( n = 20); ( G – I ) on day 6 after infection ( n = 24). ( J – M ) TFH cells were defined as germinal center (GC) TFH (CD45 + CD3 + CD4 + CD44 + PD1 high CXCR5 + ) and non-GC TFH cells (CD45 + CD3 + CD4 + CD44 + PD1-low CXCR5 + ) by flow cytometry. ( J and K ) TFH subsets on day 0 prior to infection ( n = 10); ( L and M ) TFH subsets on day 6 after infection( n = 28). A 2-tailed t test for normally distributed data and a Mann-Whitney test for nonnormally distributed data were used. * P < 0.05, ** P < 0.01, and *** P < 0.001. The error bar indicates SEM.

    Article Snippet: Cells were washed and resuspended in Foxp3 Fix/Perm WorkinFg Solution (ebiosciences, 00-5523-00) and incubated overnight at 4°C.

    Techniques: Infection, Flow Cytometry, MANN-WHITNEY